InVivoMAb anti-mouse PD-1 (CD279)

货号 #BE0146
克隆号:
RMP1-14
反应种属:
Mouse

¥1,712 - ¥46,336

选择参数
  • 100 mg - ¥46,336
  • 50 mg - ¥33,152
  • 25 mg - ¥22,480
  • 5 mg - ¥6,576
  • 1 mg - ¥1,712
  • 定制包装(请询价)

产品详情

The RMP1-14 monoclonal antibody reacts with mouse PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1’s structure includes a ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 and PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T-cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance and prevention of autoimmune disease in mice as PD-1 knockout animals show dilated cardiomyopathy, splenomegaly, and loss of peripheral tolerance. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being explored as cancer treatments. Like the J43 antibody the RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1.

参数

同种型 Rat IgG2a, κ
推荐的同型对照 InVivoMAb rat IgG2a isotype control, anti-trinitrophenol
推荐稀释缓冲液 InVivoPure pH 7.0 Dilution Buffer
免疫原 Syrian Hamster BKH cells transfected with mouse PD-1 cDNA
Reported Applications in vivo blocking of PD-1/PD-L signaling
形式 PBS, pH 7.0 Contains no stabilizers or preservatives
内毒素 <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay
纯化 >95% Determined by SDS-PAGE
无菌性 0.2 μM filtration
生产 Purified from tissue culture supernatant in an animal free facility
提纯 Protein G
RRID AB_10949053
分子量 150 kDa
保存 The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
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