RecombiMAb anti-mouse/human EGFR (domain III)
货号 #CP107
克隆号:
EMab-88-CP107
反应种属:
Mouse
￥5,824 - ￥78,848
产品详情
The EMab-88-CP107 monoclonal antibody is a recombinant, Fc-engineered chimeric version of the original EMAb-88 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Mouse IgG1, κ to Mouse IgG2a, κ for use in murine models. This antibody has an effector function competent Fc domain allowing for activation of Fcγ receptors (FcγRs) to trigger antibody‑dependent cellular cytotoxicity (ADCC), antibody‑dependent cellular phagocytosis (ADCP), complement‑dependent cytotoxicity (CDC) and opsonization to promote target cell depletion. The mouse IgG2a isotype demonstrates strong effector functions due to potent interaction with mFcγRIV, which is functionally similar to the FcγRIIIa receptor involved in human ADCC. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. The EMab-88 monoclonal antibody reacts with domain III of the mouse and human epidermal growth factor receptor (EGFR), also known as ERBB, ERBB1, and HER1. EGFR is a transmembrane glycoprotein and a member of the ErbB family of receptor protein tyrosine kinases (RTKs). The extracellular region of EGFR has four domains, wherein domains I and III contain the ligand-binding site. EGFR ligands include EGF, AREG, TGF-α, epiregulin (EREG), epigen, betacellulin, and heparin-binding EGF. Upon ligand binding, EGFR dimerizes, and its activation through the autophosphorylation of its cytoplasmic domain triggers several downstream signaling pathways, including RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC-γ-PKC, and STATs, which help the cell respond accurately to extracellular signals. EGFR is expressed ubiquitously, and in various tissues, it plays critical roles in the developmental, physiological, and regenerative processes. EGFR’s aberrant activation is a common feature of various malignancies. Using monoclonal antibodies to target the EGFR extracellular region has shown promising results in anti-cancer research, and currently approved therapeutic antibodies such as cetuximab, panitumumab, nimotuzumab, and necitumumab bind to domain III of EGFR, which directly blocks EGF-EGFR binding.参数
| 同种型 | Mouse IgG2a, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb mouse IgG2a isotype control, unknown specificity |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Human EGFR (ErbB1) |
| Reported Applications | Flow cytometry ELISA For details on in vivo applications, please contact technicalservice@bioxcell.com |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | ≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | ≥95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from mammalian cell supernatant in an animal-free facility |
| 提纯 | Protein G |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
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Recommended Isotype Control(s)
RecombiMAb mouse IgG2a isotype control, unknown specificity
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
