RecombiMAb anti-mouse VEGFR-2 (LALA-PG)
货号 #CP089
克隆号:
DC101-CP089
反应种属:
Mouse
￥5,824 - ￥154,539
产品详情
The DC101-CP089 monoclonal antibody is a recombinant, Fc-engineered chimeric version of the original DC101 antibody. The variable domain sequences are identical but the constant region sequences have been switched from rat IgG1to mouse IgG2a, κ for use in murine models. Additionally, DC101-CP089 contains LALA-PG mutations in the heavy chain Fc fragment rendering it unable to bind endogenous murine Fcγ receptors or C1q to induce antibody-dependent, cell-mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC) while preserving VEGFR-2 blockade. This modification prevents off-target depletion of VEGFR-2-expressing endothelial cells via immune effector functions, focusing activity on signaling inhibition. Species-matched chimeric antibodies demonstrate reduced immunogenicity and formation of anti-drug antibodies (ADAs) compared to xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies The DC101-CP089 monoclonal antibody reacts with mouse VEGFR-2 (vascular endothelial growth factor receptor 2) also known as CD309, KDR, and Flk-1. VEGFR-2 is a member of the tyrosine protein kinase family. Upon binding to its ligand VEGF, VEGFR-2 plays key roles in vascular development and permeability. The DC101 antibody has been shown to inhibit VEGFR-2 signaling in vivo.参数
| 同种型 | Mouse IgG2a, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Mouse VEGFR-2-SEAPs soluble receptor |
| Reported Applications | in vivo blocking of VEGF/VEGFR-2 signaling* in vitro blocking of VEGFR signaling* Western blot *Reported for the original rat IgG1 DC101 antibody |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | ≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | ≥95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from mammalian cell supernatant in an animal-free facility |
| 提纯 | Protein G |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
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Recommended Isotype Control(s)
RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
