InVivoMAb anti-mouse CD20
货号 #BE0356
克隆号:
MB20-11
反应种属:
Mouse
￥1,851 - ￥48,376
产品详情
The MB20-11 monoclonal antibody reacts with mouse CD20. CD20 is a B cell-specific 33-37 kDa transmembrane protein which is also known as B-lymphocyte antigen, B1, and Bp35. CD20 is expressed on the surface of immature and mature B cells and their malignant counterparts. CD20 plays roles in intracellular calcium regulation and B cell activation and is critical for an optimal B cell immune response against T-independent antigens. B cell depleting antibodies are valuable tools for studying B cell biology and developing therapies for autoimmune diseases and malignancies. The MB20-11 antibody rapidly and effectively depletes B cells via antibody-dependent cellular cytotoxicity (ADCC) through engaging Fcγ receptors on monocytes and other effector cells. Isotype-specific interactions contribute significantly to the effectiveness of CD20 mAbs in vivo, with IgG2a/c mAbs having greater potency than IgG1 or IgG2b. IgG2a and IgG2c isotypes are indistinguishable in their specificities to murine FcγR. Mouse strains such as C57Bl/6, C57Bl/10, SJL, and NOD mice possesses the Igh1-b allele resulting in only the expression of IgG2c. However, mouse strains such as BALB/c and Swiss Webster mice possess the Igh1-a allele which results in only the expression of IgG2a. It is important to consider matching the Ig-haplotype of the receiving mice to the isotype of the injected antibody to avoid eliciting an undesired immune response. Bio X Cell is pleased to also offer recombinant MB20-11-CP062. This monoclonal antibody has variable domain sequences identical to MB20-11 but the constant region has been converted from mouse IgG2c to mouse IgG2a for use in mice with the Igh-1a allele. Additionally, the highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. 1: Uchida, Junji et al. “The innate mononuclear phagocyte network depletes B lymphocytes through Fc receptor-dependent mechanisms during anti-CD20 antibody immunotherapy.” The Journal of experimental medicine vol. 199,12 (2004): 1659-69. doi:10.1084/jem.20040119 2: Xiu, Yan et al. “B lymphocyte depletion by CD20 monoclonal antibody prevents diabetes in nonobese diabetic mice despite isotype-specific differences in Fc gamma R effector functions.” Journal of immunology (Baltimore, Md. : 1950) vol. 180,5 (2008): 2863-75. doi:10.4049/jimmunol.180.5.2863 3: Hamaguchi, Yasuhito et al. “Antibody isotype-specific engagement of Fcgamma receptors regulates B lymphocyte depletion during CD20 immunotherapy.” The Journal of experimental medicine vol. 203,3 (2006): 743-53. doi:10.1084/jem.20052283 4: Zhang, Zhiping et al. “Possible allelic structure of IgG2a and IgG2c in mice.” Molecular immunology vol. 50,3 (2012): 169-71. doi:10.1016/j.molimm.2011.11.006参数
| 同种型 | Mouse IgG2c, κ |
|---|---|
| 推荐的同型对照 | InVivoMAb mouse IgG2c isotype control, anti-dengue virus |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Mouse CD20-GFP transfected 300.19 cells |
| Reported Applications | in vivo B cell depletion Western blot |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | ≤1EU/mg (≤0.001EU/μg) Determined by LAL assay |
| 纯化 | ≥95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 提纯 | Protein A |
| RRID | AB_2894775 |
| 分子量 | 150 kDa |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
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Recommended Isotype Control(s)
InVivoMAb mouse IgG2c isotype control, anti-dengue virus
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
