InVivoMAb anti-mouse CXCL10 (IP-10)
货号 #BE0440
克隆号:
1F11
反应种属:
Mouse
￥1,851 - ￥48,376
产品详情
The 1F11 monoclonal antibody reacts with the pro-inflammatory cytokine C-X-C motif chemokine ligand 10 (CXCL10), also known as 10 kDa interferon gamma-induced protein (gamma-IP10 or IP-10), Crg2, Ifi10, Inp10, and Scyb10. CXCL10 is a secreted protein that is mainly produced by cancer cells, endothelial cells, fibroblasts, and monocytes in response to IFN-gamma secretion. CXCL10 binds its only receptor, C-X-C motif chemokine receptor 3 (CXCR3), to activate several downstream pathways, including Src, PI3K-AKT, Erk1/2, and MAKP signaling. The CXCL10-CXCR3 axis activates G protein-mediated signaling, leading to the recruitment of activated Th1 lymphocytes to inflammatory sites (e.g., tumors, brain injury, and viral or Toxoplasma gondii infections) through a phospholipase C-dependent downstream pathway. In tumors, CXCL10-CXCR3 regulates immune cell activation, differentiation, and migration to promote anti-tumor immunity through paracrine signaling. The tumor-derived CXCL10 molecules, on the contrary, interact with CXCR3, thereby inducing cancer cell proliferation, tumor angiogenesis, and other pro-cancerous effects. During brain injury, the CXCL10/CXCR3 axis is involved in the activation and recruitment of mircoglia (i.e., the CNS’ resident macrophage population) to the lesion sites, which is an essential element for neuronal reorganization. The 1F11 monoclonal antibody has been extensively used for neutralization of CXCL10 (in vitro and in vivo) and for inhibition of T cell recruitment in vivo in a range of inflammatory disease models. The 1F11 antibody is specific for mouse CXCL10/IP-10, and it does not bind its closest known homolog CXCL9/Mig or other chemokines, including MIP-1 alpha, MIP-1beta, SDF-1, KC, TCA-3, RANTES, eotaxin, MCP-1, MCP-3, and MCP-5 (Khan et al. 2000, Immunity, 12: 483–94). This antibody binds soluble CXCL10, but it does not bind the membrane-bound form of CXCL10, i.e., the glycosaminoglycan (GAG)-bound chemokine present on endothelial cell surfaces.参数
| 同种型 | Armenian hamster IgG |
|---|---|
| 推荐的同型对照 | InVivoMAb polyclonal Armenian hamster IgG |
| 推荐稀释缓冲液 | InVivoPure pH 6.0T Dilution Buffer |
| 免疫原 | Recombinant E. coli-produced murine IP-10 |
| Reported Applications | in vitro neutralization of CXCL10 in vivo neutralization of CXCL10 in vivo inhibition of T cell recruitment Functional assays ELISA Western blot |
| 形式 | PBS, pH 6.0 with 0.01% TWEEN® 80 Contains no stabilizers or preservatives |
| 内毒素 | ≤1EU/mg (≤0.001EU/μg) Determined by LAL assay |
| 纯化 | ≥95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 提纯 | Protein A |
| 分子量 | 150 kDa |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
推荐产品
-
Recommended Isotype Control(s)
InVivoMAb polyclonal Armenian hamster IgG
-
Recommended Dilution Buffer
InVivoPure pH 6.0T Dilution Buffer
