InVivoMAb anti-mouse EGFR (domain I)
货号 #BE0465
克隆号:
EMab-300
反应种属:
Mouse
￥1,851 - ￥48,376
产品详情
The EMab-300 monoclonal antibody reacts with domain I of the mouse epidermal growth factor receptor (EGFR), also known as ERBB, ERBB1, and HER1. EGFR is a transmembrane glycoprotein and a member of the ErbB family of receptor protein tyrosine kinases (RTKs). The extracellular region of EGFR has four domains, wherein domains I and III contain the ligand-binding site. EGFR ligands include EGF, AREG, TGF-α, epiregulin (EREG), epigen, betacellulin, and heparin-binding EGF. Upon ligand binding, EGFR dimerizes, and its activation through the autophosphorylation of its cytoplasmic domain triggers several downstream signaling pathways, including RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC-γ-PKC, and STATs, which help the cell respond accurately to extracellular signals. EGFR is expressed ubiquitously, and in various tissues, it plays critical roles in the developmental, physiological, and regenerative processes. EGFR’s aberrant activation is a common feature of various malignancies. Using monoclonal antibodies to target the EGFR extracellular region has shown promising results in anti-cancer research, and currently approved therapeutic antibodies such as cetuximab, panitumumab, nimotuzumab, and necitumumab bind to EGFR's domain III, which, together with domain I, is well known to facilitate EGF-EGFR binding.参数
| 同种型 | Rat IgG1, κ |
|---|---|
| 推荐的同型对照 | InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | mEGFR over-expressing LN229 cells |
| Reported Applications | Flow cytometry For details on in vivo applications, please contact technicalservice@bioxcell.com |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | ≤1EU/mg (≤0.001EU/μg) Determined by LAL assay |
| 纯化 | ≥95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtered |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 提纯 | Protein G |
| 分子量 | 150 kDa |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
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Recommended Isotype Control(s)
InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
