RecombiMAb anti-mouse CD19
货号 #CP118
克隆号:
1D3-CP118
反应种属:
Mouse
￥5,824 - ￥154,539
产品详情
The 1D3-CP118 monoclonal antibody is a chimeric version of the original 1D3 antibody. The variable domain sequences are identical but the constant region sequences have been switched from rat IgG2a to mouse IgG2a. CP118 antibody has an effector function competent Fc domain allowing for activation of Fcγ receptors (FcγRs) to trigger antibody‑dependent cellular cytotoxicity (ADCC), antibody‑dependent cellular phagocytosis (ADCP), complement‑dependent cytotoxicity (CDC) and opsonization to promote target cell depletion. The mouse IgG2a isotype demonstrates strong effector functions due to potent interaction with mFcγRIV, which is functionally similar to the FcγRIIIa receptor involved in human ADCC. Species-matched chimeric antibodies result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. The 1D3-CP118 monoclonal antibody reacts with mouse CD19, a B cell-specific 95 kDa transmembrane glycoprotein of the immunoglobulin superfamily. CD19 contains two extracellular immunoglobulin-like domains and an extensive cytoplasmic tail. It functions as a positive regulator of B-cell receptor signaling in conjunction with CD21 and CD81. CD19 is highly expressed on autoreactive B cells and in most lymphomas and leukemias including some early B-cell malignancies that do not express CD20. For these reasons CD19 is an attractive target for the immunotherapy of lymphoproliferative disorders and autoimmune disease.参数
| 同种型 | Mouse IgG2a, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb mouse IgG2a isotype control, unknown specificity |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | K562 cells expressing the extracellular domain of mouse CD19 |
| Reported Applications | in vivo B cell depletion* in vivo CD19 neutralization* in vitro B cell negative selection* Flow cytometry* *Reported for the original rat IgG2a 1D3 antibody |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | ≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | ≥95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from mammalian cell supernatant in an animal-free facility |
| 提纯 | Protein G |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
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Recommended Isotype Control(s)
RecombiMAb mouse IgG2a isotype control, unknown specificity
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
