RecombiMAb anti-mouse CD40 (LALA-PG)
货号 #CP088
克隆号:
FGK4.5-CP088
反应种属:
Mouse
￥5,824 - ￥154,539
产品详情
The FGK4.5-CP088 monoclonal antibody is a recombinant, Fc-engineered chimeric version of the original FGK4.5 antibody. The variable domain sequences are identical but the constant region sequences have been switched from rat IgG2a to mouse IgG2a, κ for use in murine models. Species-matched chimeric antibodies demonstrate reduced immunogenicity and formation of anti-drug antibodies (ADAs) compared to xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. Additionally, FGK4.5-CP088 contains LALA-PG mutations in the heavy chain Fc fragment rendering it unable to bind endogenous murine Fcγ receptors or C1q to induce antibody-dependent, cell-mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC). This modification facilitates the comparison between FcγR-independent and FcγR-dependent agonistic activity. Studies have demonstrated anti-CD40 antibodies lacking Fc effector functions are better tolerated than FcγR-binding competent antibody variants [1]. 1. Lang, Isabell et al. “FcγRs and Their Relevance for the Activity of Anti-CD40 Antibodies.” International journal of molecular sciences vol. 23,21 12869. 25 Oct. 2022, doi:10.3390/ijms232112869参数
| 推荐的同型对照 | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
|---|---|
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Recombinant mouse CD40 fusion protein |
| Reported Applications | in vivo CD40 activation* in vitro B cell stimulation/activation* *Reported for the original rat IgG2a FGK4.5 antibody |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | ≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | ≥95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from mammalian cell supernatant in an animal-free facility |
| 提纯 | Protein G |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
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Recommended Isotype Control(s)
RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
