RecombiMAb anti-mouse CTLA-4 (CD152) (LALA-PG)
货号 #CP008
克隆号:
9D9-CP008
反应种属:
Mouse
￥5,360
产品详情
The 9D9-CP008 monoclonal antibody is a chimeric version of the original 9D9 antibody. The variable domain sequences are identical to the original 9D9 but the constant region sequences have been switched from mouse IgG2b to mouse IgG2a. The 9D9-CP008 antibody also contains a LALA-PG mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. 9D9-CP008 reacts with mouse CTLA-4 (cytotoxic T lymphocyte antigen-4) also known as CD152. CTLA-4 is a 33 kDa cell surface receptor encoded by the Ctla4 gene that belongs to the CD28 family of the Ig superfamily. CTLA-4 is expressed on activated T and B lymphocytes. CTLA-4 is structurally similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to the B7 family members B7-1 (CD80) and B7-2 (CD86). Upon ligand binding, CTLA-4 negatively regulates cell-mediated immune responses. CTLA-4 plays roles in induction and/or maintenance of immunological tolerance, thymocyte development, and regulation of protective immunity. The critical role of CTLA-4 in immune down-regulation has been demonstrated in CTLA-4 deficient mice, which succumb at 3-5 weeks of age due to the development of a lymphoproliferative disease. CTLA-4 is among a group of inhibitory receptors being explored as cancer treatment targets through immune checkpoint blockade. The 9D9 antibody has been shown to promote T cell co-stimulation by blocking CTLA-4 binding to the B7 co-receptors, allowing for CD28 binding.参数
| 同种型 | Mouse IgG2a, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Not available or unknown |
| Reported Applications | in vivo CTLA-4 neutralization* Western blot* *Reported for the original mouse IgG2b 9D9 antibody |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | <1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | >95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from HEK293 cell supernatant in an animal free facility |
| 提纯 | Protein A |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
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Recommended Isotype Control(s)
RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
