InVivoMAb anti-human mesothelin
货号 #BE0405
克隆号:
YP218
反应种属:
Human
￥1,792 - ￥46,736
产品详情
The YP218 monoclonal antibody reacts with a very rare epitope in the C-terminal end (region III, within amino acids 525 to 560) close to the tumor cell surface of human mesothelin (MSLN). MSLN has limited expression in normal tissues, while its expression gets upregulated in many solid tumors, including mesothelioma, epithelial ovarian cancer, pancreatic adenocarcinoma, lung cancer, uterine cancer, and cholangiocarcinoma. MSLN interacts with MUC16/CA125, and it regulates the processes of cell proliferation, growth, and adhesion. Human MSLN is synthesized as a 71-kDa precursor that translocates to the cell surface, where it is cleaved into 31-kDa megakaryocyte potentiating factor (MPF) and 40-kDa mature MSLN. The YP218 antibody has been reported for use in combination with clone YP223 in sandwich ELISAs for the detection of soluble MSLN in human mesothelioma serum samples. The YP218 antibody sequence was used for generating MSLN region III (membrane-proximal region)-targeting Meso3 CAR T cells, which displayed stronger antitumor responses in vivo than Meso1 CAR T cells (membrane-distal region) in gastric cancer NSG mouse models and large ovarian tumors. A humanized version of YP218 (i.e., hYP218) has been generated and used to make an antibody-drug conjugate (ADC) called NAV-001-PNU that is highly effective against MUC16/CA125 HIO-positive tumor cell lines as well as several patient-derived xenografts (PDX). The humanized version of YP218 was also conjugated to a photosensitizing phthalocyanine dye, IRDye 700DX NHS ester. The IR700-hYP218 conjugate caused rapid cell death of MSLN-expressing A431/H9 cell lines in vitro and significant inhibition of tumor growth with improved survival in vivo (upon exposure to near-infrared light). BE0405 is the original rabbit IgG version of YP218 and not the humanized version.参数
| 同种型 | Rabbit IgG |
|---|---|
| 推荐的同型对照 | InVivoMAb polyclonal rabbit IgG |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| Reported Applications | in vitro photoimmunotherapy* in vivo photoimmunotherapy* Chimeric antigen receptor construction Antibody-drug conjugate synthesis* Functional assays Immunohistochemistry (paraffin) Flow cytometry ELISA *Use of humanized YP218 (see "References") |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay |
| 纯化 | >95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtered |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 提纯 | Protein A |
| 分子量 | 150 kDa |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
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Recommended Isotype Control(s)
InVivoMAb polyclonal rabbit IgG
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
