InVivoSIM anti-human amyloid beta (Donanemab Biosimilar)
货号 #SIM0048
克隆号:
Donanemab
反应种属:
Human
￥2,448 - ￥84,656
产品详情
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Donanemab, making it ideal for research use. Donanemab is a humanized IgG1κ anti-human APP antibody, and it specifically reacts with abeta 42 (Aβ42), a pyroglutamate form of Aβ that is aggregated in amyloid plaques during the pathogenesis of Alzheimer’s disease (AD). Aβ42 results from an aberrant processing of amyloid-β protein precursor (APP or AβPP). The initial cleavage of APP is caused by either α- secretase or β-secretase (or BACE1). sAPPα and the α-C-terminal fragment (α-CTF or APP-C83) are produced by α-secretase cleavage of APP, whereas sAPPβ and the β-C-terminal fragment (β-CTF or APP-C99) are produced by β-secretase cleavage. γ-secretase further cleaves C83 and C99, yielding the P3 peptide and Aβ, respectively. Depending on the location of the γ-secretase cleavage, Aβ can have a length of 37-43 amino acids in its peptide sequence. The two main Aβ species are Aβ42 and Aβ40. In early AD pathology, Aβ species play a critical role by seeding toxic oligomeric species with Aβ42 being more preferentially depositing in amyloid plaques than Aβ40. Donanemab specifically recognizes Aβ p3-42 (Aβ42) and it is reported to impede the progression of AD by clearing plaques through microglial-mediated phagocytosis in mice without causing microhemorrhages. Donanemab biosimilar antibodies are commonly used for in vitro and in vivo studies involving Alzheimer's disease and Down syndrome experimental models.参数
| 同种型 | Human IgG1, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Human APP |
| Reported Applications | in vivo functional assays in vitro functional assays ELISA Western blot |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | <0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | >95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
推荐产品
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Recommended Isotype Control(s)
RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
