InVivoSIM anti-human CXCL10 (IP-10) (Eldelumab Biosimilar)
货号 #SIM0076
克隆号:
Eldelumab
￥2,527 - ￥87,620
产品详情
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, eldelumab, making it ideal for research use. Eldelumab is a humanized IgG1, k monoclonal antibody that specifically binds the pro-inflammatory cytokine C-X-C motif chemokine ligand 10 (CXCL10), also known as 10 kDa interferon gamma-induced protein (gamma-IP10 or IP-10). Importantly, the Fc of the eldelumab antibody is engineered to replace lysine (K) at position 214 with arginine (R) to reduce the Fc effector function (i.e., to lower the ADCC or CDC activity of the antibody). CXCL10 is a secreted protein that is mainly produced by cancer cells, endothelial cells, fibroblasts, and monocytes in response to IFN-gamma secretion. CXCL10 binds its only receptor, CXCR3, to activate Src, PI3K-AKT, Erk1/2, MAKP, and other downstream pathways. The CXCL10-CXCR3 axis activates G protein-mediated signaling, leading to the recruitment of activated Th1 lymphocytes to inflammatory sites, including tumors, brain injury, and viral or Toxoplasma gondii infections. In tumors, CXCL10-CXCR3 regulates immune cell activation, differentiation, and migration to promote anti-tumor immunity through paracrine signaling. The tumor-derived CXCL10 molecules, on the contrary, interact with CXCR3, thereby inducing cancer cell proliferation, tumor angiogenesis, and other pro-cancerous effects. During brain injury, the CXCL10/CXCR3 axis is involved in the activation and recruitment of microglia to the lesion sites, which is an essential element for neuronal reorganization. In experiments involving CXCL10-stimulated human PBMCs, NF-κB-overexpressing human umbilical vein endothelial cells (HUVECs), and CXCR3-expressing cells, in vitro treatment with eldelumab antibody is reported to neutralize the CXCL10 activity.参数
| 同种型 | Human IgG1, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb human IgG1 (K214R/L234F/L235E/P331S) isotype control, anti-hen egg lysozyme |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Human CXCL10 |
| Reported Applications | in vivo functional assays in vitro functional assays Flow cytometry ELISA |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | ≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | ≥95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 提纯 | Protein A |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
推荐产品
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Recommended Isotype Control(s)
RecombiMAb human IgG1 (K214R/L234F/L235E/P331S) isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
