InVivoSIM anti-human EGFR (Panitumumab Biosimilar)
货号 #SIM0050
克隆号:
Panitumumab
反应种属:
Human
￥2,448 - ￥84,656
产品详情
This biosimilar antibody uses the same variable regions as the therapeutic antibody Panitumumab, making it ideal for research use. Panitumumab is a fully humanized IgG2κ monoclonal antibody that reacts with epidermal growth factor receptor (EGFR), a member of the HER tyrosine kinase growth factor receptor family. EGFR binds to EGF family ligands, which sets off a number of signaling pathways that turn extracellular cues into the right responses from cells. EGFR binding with its known ligands including EGF, TGF, AREG, EPGN, BTC, epiregulin (EREG), and heparin-binding EGF triggers receptor homo- and/or heterodimerization, autophosphorylation, and recruitment of adapter proteins like GRB2, thereby activating complex downstream signaling pathways including RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC, STAT modules, and NF-κB. EGFR directly phosphorylates RGS16 for coupling the EGFR signaling to GPCR signaling and also phosphorylates MUC1 for regulating SRC and beta-catenin signaling. EGFR is known to play critical roles in cellular proliferation, angiogenesis, and cell survival. Several studies have shown that agonist antibodies such as Panitumumab bind EGFR more strongly than EGFR's natural partners. Preclinical studies have shown that panitumumab binds to EGFR and stops ligand-induced receptor autophosphorylation and activation of receptor-associated kinases, thereby causing the inhibition of cell growth, induction of apoptosis, downregulation of the production of pro-inflammatory cytokines and vascular growth factors, and internalization of the EGFR. Studies with in vitro and in vivo experimental models have demonstrated that the neutralization of EGFR with Panitumumab leads to cell cycle arrest and inhibition of tumor growth.参数
| 同种型 | Human IgG2, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb human IgG2 isotype control, anti-hen egg lysozyme |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Human EGFR (ErbB1) |
| Reported Applications | in vivo neutralization of human EGFR in vitro neutralization of human EGFR ELISA Western blot |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | <0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | >95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
推荐产品
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Recommended Isotype Control(s)
RecombiMAb human IgG2 isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
