InVivoSIM anti-human IL-1β (Gevokizumab Biosimilar)
货号 #SIM0049
克隆号:
Gevokizumab
反应种属:
Human
￥2,448 - ￥84,656
产品详情
This biosimilar antibody uses the same variable regions as the therapeutic antibody Gevokizumab, making it ideal for research use. Gevokizumab is a fully humanized monoclonal antibody that reacts with human IL-1β, a potent pro-inflammatory cytokine produced primarily by monocytes. Inflammatory signals, such as LPS, stimulate the synthesis and promote the accumulation of cytosolic stores of pro-IL1 β, and after inflammasome assembly-mediated CASP1 activation, pro-IL1 is processed to generate active cytokine for secretion. IL-1β is an important mediator of the inflammatory response and is involved in a variety of cellular activities, including cell proliferation, differentiation, apoptosis, and T and B lymphocyte activation, neutrophil influx and activation, cytokine production, antibody production, fibroblast proliferation, and the synthesis of collagen as well as prostaglandins. IL-1β promotes Th17 differentiation of T-cells and also shows synergism with IL-12 to induce IFN-γ synthesis from Th1 cells. The Gevokizumab antibody binds a unique IL-1β epitope that is proximal to, but does not overlap with, the receptor/ligand interface, and this interaction reduces the binding of IL-1 to its receptor, i.e., IL-1 receptor type I (IL-1RI or CD121a). Gevokizumab neutralizes the human IL-1β by binding to it, and this antibody is used in preclinical research involving in vitro and in vivo experimental models of type 1 diabetes mellitus, inflammation, rheumatoid arthritis, cardiovascular biology, and cancer immunotherapy.参数
| 同种型 | Human IgG2, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb human IgG2 isotype control, anti-hen egg lysozyme |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Human IL-1β |
| Reported Applications | in vivo neutralization of human IL1β in vitro neutralization of human IL1β ELISA Western blot Flow cytometry |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | <0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | >95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
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Recommended Isotype Control(s)
RecombiMAb human IgG2 isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
