InVivoSIM anti-human Tissue Factor (CD142) (Tisotumab Biosimilar)
货号 #SIM0084
克隆号:
Tisotumab
反应种属:
Human
￥2,527 - ￥87,620
产品详情
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Tisotumab, making it ideal for research use. Tisotumab is a humanized IgG1 monoclonal antibody that reacts with human and monkey tissue factor (TF), also known as CD142, coagulation factor III (F3), and thromboplastin. TF is a single-pass transmembrane glycoprotein that is essential for initiating blood coagulation by forming a complex with circulating factor VII (F7) or serum prothrombin conversion accelerator (SPCA). This interaction converts the factor VII to VIIa, which follows the TF:VIIa complex-mediated activation of factors IX or X through proteolysis. Recent research has explored the involvement of CD142 in additional cellular signaling pathways responsible for tumor growth, e.g., mitogen-activated protein kinase (MAPK), phosphatidylinositol-3-kinase AKT (PI3K-AKT), and Wnt signaling pathways. TF shows aberrant expression in some tumors, and owing to its internalizing capacity, an in vivo study with patient-derived xenograft models of solid tumors has explored the potential of a monomethyl auristatin E (MMAE)-based antibody-drug conjugate (ADC) targeting TF for its anti-cancer activity. The tisotumab biosimilar is an antibody portion of an ADC called tisotumab vedotin, which is known for targeting the TF antigen, thereby directly delivering the cytotoxic microtubule-disrupting agent MMAE to cancer cells.参数
| 同种型 | Human IgG1, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb human IgG1 (K214R/L234F/L235E/P331S) isotype control, anti-hen egg lysozyme |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Human Tissue Factor (CD142) |
| Reported Applications | Antibody-drug conjugate synthesis In vivo functional assays in vitro functional assays Flow cytometry ELISA |
| 形式 | pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | ≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | ≥95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 提纯 | Protein A |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
推荐产品
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Recommended Isotype Control(s)
RecombiMAb human IgG1 (K214R/L234F/L235E/P331S) isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
