InVivoSIM anti-human TROP-2 (Sacituzumab Biosimilar)
货号 #SIM0051
克隆号:
Sacituzumab
反应种属:
Human
￥2,448 - ￥84,656
产品详情
This biosimilar antibody uses the same variable regions as the therapeutic antibody Sacituzumab, making it ideal for research use. Sacituzumab is a fully humanized IgG1κ monoclonal antibody that reacts with human trophoblastic cell surface antigen 2 (TROP-2), an antigen linked to cancer. TROP-2 is a cell surface receptor that spans the cellular membrane with an extracellular, transmembrane, and intracellular domain, along with a cytoplasmic tail essential for signaling. The ligands of TROP-2 include claudin-1, claudin-7, cyclin D1, and potentially IGF-1. TROP-2 upregulates EpCAM-triggered cell signaling and requires RIP to regulate efficient growth and division of cancer cells. Several proteins, including RIP, TACE, γ-secretase, Presenilin 1 (PS-1) and PS-2, facilitate TROP-2’s cleavage within the transmembrane domain, and this cleavage produces TROP-2 extracellular domain (ECD) and intracellular domain (ICD) fragments. TROP-2 is considered a cellular marker of trophoblastic stem cells, and this glycoprotein is known to transduce calcium signals. Normal epithelial cells express TROP-2 at a low level, while many tumors (such as glioblastoma, pancreatic carcinoma, and all breast cancer subtypes, especially triple-negative breast cancer) express it at a high level. The critical role of TROP-2 in multiple signaling pathways often links to the proliferation, invasion, and metastasis of tumors. The tumor cell-specific expression of TROP-2 makes it an ideal target for cancer immunotherapy. Several recent studies have used Sacituzumab-based antibody-drug conjugates (ADC), namely Sacituzumab Govitecan (IMMU-132) that contains Sacituzumab conjugated to SN-38 (the active metabolite of irinotecan), utilizing a pH hydrolysable linker CL2A for facilitating SN-38 release to the cancer cells in in vitro and in vivo preclinical studies.参数
| 同种型 | Human IgG1, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb human IgG1 (K214R/L234F/L235E/P331S) isotype control, anti-hen egg lysozyme |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Human TROP-2 |
| Reported Applications | in vitro functional assays in vivo functional assays Antibody-drug conjugate synthesis ELISA Western blot Flow cytometry |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | <0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | >95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
推荐产品
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Recommended Isotype Control(s)
RecombiMAb human IgG1 (K214R/L234F/L235E/P331S) isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
