InVivoMAb anti-canine PD-L1 (B7-H1)
货号 #BE0390
克隆号:
JC071
反应种属:
Canine
¥1,712 - ¥44,512
产品详情
The JC071 monoclonal antibody reacts with canine PD-L1 (programmed death ligand 1) also known as B7-H1 or CD274. PD-L1 is a 40 kDa type I transmembrane protein that belongs to the B7 family of the Ig superfamily. PD-L1 is expressed on T lymphocytes, B lymphocytes, NK cells, dendritic cells, as well as IFNγ stimulated monocytes, epithelial cells, and endothelial cells. PD-L1 binds to its receptor, PD-1, found on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Engagement of PD-L1 with PD-1 leads to inhibition of TCR-mediated T cell proliferation and cytokine production. PD-L1 is thought to play an important role in tumor immune evasion. Induced PD-L1 expression is common in many tumors and results in increased resistance of tumor cells to CD8 T cell mediated lysis. The JC071 antibody can detect PD-L1 on canine tissues by flow cytometry and Western blot.参数
同种型 | Mouse IgG1, κ |
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推荐的同型对照 | InVivoMAb mouse IgG1 isotype control, unknown specificity |
推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
免疫原 | Recombinant canine PD-L1-Ig fusion protein |
Reported Applications | in vitro PD-L1 blockade Functional assays Western blot Flow cytometry |
形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
内毒素 | <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay |
纯化 | >95% Determined by SDS-PAGE |
无菌性 | 0.2 μM filtered |
生产 | Purified from tissue culture supernatant in an animal free facility |
提纯 | Protein G |
分子量 | 150 kDa |
保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |