InVivoMAb anti-rat CD25 (IL-2Rα)

货号 #BE0453
克隆号:
OX-39
反应种属:
Rat

¥1,792 - ¥46,736

选择参数
  • 100 mg - ¥46,736
  • 50 mg - ¥33,024
  • 25 mg - ¥21,936
  • 5 mg - ¥6,560
  • 1 mg - ¥1,792
  • 定制包装(请询价)

产品详情

The OX-39 monoclonal antibody recognizes the 55 kDa low-affinity interleukin-2 receptor α chain (IL-2R α), also known as CD25 and OX-39 antigen. CD25 binds interleukin-2 (IL-2) with low affinity, but after interacting with IL-2Rβ (CD122) and IL-2Rγc (CD132), it forms a high-affinity receptor complex for IL-2 (i.e., a high-affinity IL-2 receptor). In rats, the CD25 antigen is expressed on T blasts, antigen-activated T cells, B cells, and a subset of thymic and splenic dendritic cells. CD25 plays an important role in immune tolerance regulation by controlling the activity of regulatory T cells (Tregs), which suppress the activation and expansion of autoreactive T cells. The OX-39 antibody is commonly used as a marker for Tregs, but studies have shown a subset of Tregs not expressing CD25. This antibody is reported to weakly inhibit the IL-2-induced proliferation in vitro and allograft rejection in vivo.

参数

同种型 Mouse IgG1, κ
推荐的同型对照 InVivoMAb mouse IgG1 isotype control, unknown specificity
推荐稀释缓冲液 InVivoPure pH 7.0 Dilution Buffer
免疫原 T blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleens
Reported Applications in vivo functional assays in vitro functional assays Immunohistochemistry (paraffin) Immunohistochemistry (frozen) Immunoprecipitation Flow cytometry
形式 PBS, pH 7.0 Contains no stabilizers or preservatives
内毒素 <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay
纯化 >95% Determined by SDS-PAGE
无菌性 0.2 μM filtered
生产 Purified from cell culture supernatant in an animal-free facility
提纯 Protein G
分子量 150 kDa
保存 The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
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