InVivoMAb anti-rat CD25 (IL-2Rα)
货号 #BE0453
克隆号:
OX-39
反应种属:
Rat
￥1,792 - ￥46,736
产品详情
The OX-39 monoclonal antibody recognizes the 55 kDa low-affinity interleukin-2 receptor α chain (IL-2R α), also known as CD25 and OX-39 antigen. CD25 binds interleukin-2 (IL-2) with low affinity, but after interacting with IL-2Rβ (CD122) and IL-2Rγc (CD132), it forms a high-affinity receptor complex for IL-2 (i.e., a high-affinity IL-2 receptor). In rats, the CD25 antigen is expressed on T blasts, antigen-activated T cells, B cells, and a subset of thymic and splenic dendritic cells. CD25 plays an important role in immune tolerance regulation by controlling the activity of regulatory T cells (Tregs), which suppress the activation and expansion of autoreactive T cells. The OX-39 antibody is commonly used as a marker for Tregs, but studies have shown a subset of Tregs not expressing CD25. This antibody is reported to weakly inhibit the IL-2-induced proliferation in vitro and allograft rejection in vivo.参数
| 同种型 | Mouse IgG1, κ |
|---|---|
| 推荐的同型对照 | InVivoMAb mouse IgG1 isotype control, unknown specificity |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | T blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleens |
| Reported Applications | in vivo functional assays in vitro functional assays Immunohistochemistry (paraffin) Immunohistochemistry (frozen) Immunoprecipitation Flow cytometry |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay |
| 纯化 | >95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtered |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 提纯 | Protein G |
| 分子量 | 150 kDa |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
推荐产品
-
Recommended Isotype Control(s)
InVivoMAb mouse IgG1 isotype control, unknown specificity
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
