InVivoMAb anti-mouse/human HER2 (domain III) (CD340)
货号 #BE0461
克隆号:
H2Mab-19
反应种属:
Human
￥1,792 - ￥46,736
产品详情
The H2Mab-19 monoclonal antibody reacts with domain III of mouse and human receptor tyrosine-protein kinase erbB-2 (ERBB2), also known as human epidermal growth factor receptor 2 (HER2), CD340, or NEU. HER2 is a 185 kDa transmembrane receptor tyrosine kinase that is part of the EGFR family. The HER2 receptor contains a cytoplasmic tyrosine kinase domain, multiple cytoplasmic tyrosine residues that function as phosphorylation sites, a hydrophobic single-pass transmembrane domain, and an extensive extracellular domain (ECD) subdivided into four functional domains (I, II, III, and IV). Domains I and III facilitate ligand binding, domain II reinforces protein-protein interactions during dimerization, and domain IV stabilizes interactions between HER2 and its dimerization partner. HER2 lacks identified ligands; nonetheless, its homodimerization (HER2/HER2) and heterodimerization (EGFR/HER2, HER2/HER3, and HER2/HER4) facilitate HER2 activation, subsequently modulating downstream signaling pathways, including PI3K/Akt/mTOR and NF-kB. HER2 is essential for cell proliferation, survival, and differentiation, and in cancers, dysregulated HER2 signaling or its overexpression often correlates with aggressive tumor growth. In addition to breast cancer, HER2 is overexpressed in gastric, gastroesophageal, colorectal, lung, endometrial, and ovarian cancers. The H2Mab-19 antibody has been shown to have anti-tumor activity in HER2+ xenograft models.参数
| 同种型 | Mouse IgG2b, κ |
|---|---|
| 推荐的同型对照 | InVivoMAb mouse IgG2b isotype control, unknown specificity |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Recombinant human HER2 extracellular domain |
| Reported Applications | in vivo antitumor activity in HER2+ xenograft model in vitro induction of ADCC in HER2+ cells in vitro induction of CDC in HER2+ cells Immunohistochemistry (frozen) Flow cytometry |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay |
| 纯化 | >95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtered |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 提纯 | Protein A |
| 分子量 | 150 kDa |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
推荐产品
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Recommended Isotype Control(s)
InVivoMAb mouse IgG2b isotype control, unknown specificity
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
