RecombiMAb anti-mouse VISTA

货号 #CP053
克隆号:
13F3-CP053
反应种属:
Mouse

¥5,632 - ¥76,176

选择参数
  • 25 mg - ¥76,176
  • 5 mg - ¥21,808
  • 1 mg - ¥5,632
  • 定制包装(请询价)

产品详情

The 13F3-CP053 monoclonal antibody is a chimeric version of the original 13F3 antibody. The variable domain sequences are identical to the original 13F3 but the constant region sequences have been switched from Armenian hamster IgG to mouse IgG2a. The 13F3-CP053 antibody contains no Fc mutations just as the original hamster IgG antibody does not. The 13F3-CP053 monoclonal antibody reacts with mouse V-domain Ig suppressor of T cell activation (VISTA) also known as PD-1H and B7-H5. VISTA is a 309 aa type I transmembrane glycoprotein and a member of the Ig superfamily. VISTA is expressed on naïve and activated T cells, NK cells, macrophages, dendritic cells, and neutrophils. VISTA functions as a negative immune-checkpoint protein that suppresses T cell cytokine production and proliferation. VISTA is overexpressed by tumor-infiltrating lymphocytes, such as myeloid cells and regulatory T cells. Blockade of VISTA with the original 13F3 antibody results in delayed tumor growth in mouse models of melanoma.

参数

同种型 Mouse IgG2a, κ
推荐的同型对照 RecombiMAb mouse IgG2a isotype control, anti-hen egg lysozyme
推荐稀释缓冲液 InVivoPure pH 7.0 Dilution Buffer
免疫原 EL4 cells overexpressing mouse VISTA-RFP and then boosted with VISTA-Ig fusion protein
Reported Applications in vivo blocking of VISTA signaling* in vitro blocking of VISTA signaling* Flow cytometry* *Reported for the original Armenian hamster IgG 13F3 antibody
形式 PBS, pH 7.0 Contains no stabilizers or preservatives
内毒素 <1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay
聚合 <5% Determined by SEC
纯化 >95% Determined by SDS-PAGE
无菌性 0.2 μM filtration
生产 Purified from HEK293 cell supernatant in an animal-free facility
提纯 Protein A
分子量 150 kDa
小鼠病原体试验 Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative
保存 The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
点击关闭