InVivoMAb anti-human EGFR

货号 #BE0279
克隆号:
528
反应种属:
Human

¥1,712 - ¥44,512

选择参数
  • 100 mg - ¥44,512
  • 50 mg - ¥31,456
  • 25 mg - ¥20,896
  • 5 mg - ¥6,240
  • 1 mg - ¥1,712
  • 定制包装(请询价)

产品详情

The 528 monoclonal antibody reacts with an epitope on the extracellular domain of human EGFR (epidermal growth factor receptor) also known as ErbB-1. EGFR is a 170 kDa cell-surface receptor and belongs to the ErbB family of receptors. EGFR signaling is activated upon binding one of its ligands including epidermal growth factor (EGF), transforming growth factor α (TGF α), Amphiregulin, and heparin binding-EGF (HB-EGF). Upon activation, EGFR transitions from an inactive monomeric form to an active homodimer. This initiates several downstream signal transduction cascades including the MAPK, Akt and JNK pathways, leading to DNA synthesis and cell proliferation. EGFR overexpression or constitutive activation are associated with many cancers. For this reason, anti-EGFR monoclonal antibody mediated immunotherapies are currently being explored as cancer treatments. The 528 antibody has been reported to block EGF binding to its receptor and inhibit A431 tumor formation in nude mice.

参数

同种型 Mouse IgG2a
推荐的同型对照 InVivoMAb mouse IgG2a isotype control, unknown specificity
推荐稀释缓冲液 InVivoPure pH 7.0 Dilution Buffer
免疫原 Purified EGFR from A431 cells
Reported Applications in vitro EGFR blockade in vivo EGFR blockade in xenografts Western blot Functional assays Immunoprecipitation Immunohistochemistry (paraffin) Immunofluorescence Flow cytometry
形式 PBS, pH 7.0 Contains no stabilizers or preservatives
内毒素 <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay
纯化 >95% Determined by SDS-PAGE
无菌性 0.2 μM filtration
生产 Purified from tissue culture supernatant in an animal free facility
提纯 Protein A
RRID AB_2687802
分子量 150 kDa
保存 The antibody solution should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
点击关闭