InVivoSIM anti-human 4-1BB (Urelumab Biosimilar)
货号 #SIM0058
克隆号:
Urelumab
反应种属:
Human
￥2,448 - ￥84,656
产品详情
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Urelumab, making it ideal for research use. Urelumab reacts with 4-1BB, also known as CD137 antigen, TNF receptor superfamily member 9, or induced by lymphocyte activation. Urelumab has been engineered as a humanized IgG4 monoclonal antibody to reduce its non-specific binding to Fc receptors. Urelumab has a hinge mutation, S228P, which prevents IgG4 Fab exchange, thereby improving stability. CD137 is a type 1 membrane glycoprotein receptor that belongs to the tumor necrosis factor (TNF) superfamily, and it is expressed in lymphoid tissues. Upon activation, CD137 is expressed on the surface of several immune cells, including T cells (CD4+ and CD8+), NKT cells, regulatory T cells, eosinophils, and dendritic cells. Notably, CD137 is not expressed on the surface of resting T and B cells, as well as activated B lymphocytes, rendering it an advantageous biomarker for immunological activation. CD137L is an established ligand of CD137, and the CD137-CD137L signaling pathway augments and prolongs T-cell reactivity to alloantigens. Urelumab is an agonistic antibody that replicates cross-linking by the endogenous ligand (i.e., CD137-CD137L), and this antibody triggers a robust costimulatory signal in antigen-primed T cells, enhances the ADCC of NK cells, and boosts the T cell-mediated antitumor immunity.参数
| 同种型 | Human IgG1, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Human 4-1BB (CD137) |
| Reported Applications | in vivo 4-1BB stimulation in vitro 4-1BB stimulation Functional assays Immunoprecipitation Flow cytometry ELISA |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | <0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | >95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 提纯 | Protein A |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
推荐产品
-
Recommended Isotype Control(s)
RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
