InVivoSIM anti-human BAFF (Belimumab Biosimilar)

货号 #SIM0055
克隆号:
Belimumab
反应种属:
Human

¥2,448 - ¥84,656

选择参数
  • 100 mg - ¥84,656
  • 50 mg - ¥47,568
  • 25 mg - ¥33,088
  • 5 mg - ¥9,472
  • 1 mg - ¥2,448
  • 定制包装(请询价)

产品详情

This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Belimumab, making it ideal for research use. Belimumab is a humanized IgG1 lambda recombinant monoclonal antibody that reacts with B-cell activating factor (BAFF), also known as B lymphocyte stimulator (BLyS) or TNFSF13B. BAFF is a cytokine that belongs to the TNF ligand family and is expressed by monocytes, macrophages, dendritic cells, and lymphoid cells, including B cells and activated T cells. BAFF exists as a cell surface type II transmembrane protein and a cleaved soluble form. BAFF interacts with three distinct receptors: B-cell maturation antigen (BCMA, also known as TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI, also known as TNFRSF13B), and BAFF receptor (BAFF-R or BR3). BAFF’s expression is upregulated by IFNγ and IFNα (on monocytes and dendritic cells), and IL-10 is known to stimulate BAFF secretion by macrophages. BAFF shares amino acids and functional similarities with TNFSF13/APRIL, which also binds to TACI and BCMA receptors, but not with BR3. BAFF is involved in the stimulation of B-cell and T-cell function and the regulation of humoral immunity. BAFF promotes the survival of immature, naive, and activated B cells. Importantly, BAFF facilitates the differentiation of B cells into plasma cells. BAFF is a B cell survival factor, and its expression is often elevated in immunodeficient and autoimmune disorders, such as systemic lupus erythematosus (SLE). In experimental studies, belimumab is documented to inhibit the survival of B cells through blockade of BAFF interaction with TACI, BCMA, and BR3. The critical role of BAFF in the development of systemic lupus erythematosus (SLE) forms the basis for SLE immunotherapy with BAFF-blocking antibodies, such as belimumab.

参数

同种型 Human IgG1, λ
推荐的同型对照 RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme
推荐稀释缓冲液 InVivoPure pH 7.0 Dilution Buffer
免疫原 Human BAFF (BLYS)
Reported Applications in vivo functional assays in vitro functional assays Flow cytometry ELISA Western blot
形式 PBS, pH 7.0 Contains no stabilizers or preservatives
内毒素 <0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay
聚合 <5% Determined by SEC
纯化 >95% Determined by SDS-PAGE
无菌性 0.2 μM filtration
生产 Purified from cell culture supernatant in an animal-free facility
提纯 Protein A
分子量 150 kDa
小鼠病原体试验 Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative
保存 The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
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