InVivoSIM anti-human PD-1 (Sintilimab Biosimilar)
货号 #SIM0054
克隆号:
Sintilimab
反应种属:
Human
￥2,448 - ￥84,656
产品详情
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Sintilimab, making it ideal for research use. Sintilimab is a humanized IgG4 kappa monoclonal antibody with an S228P hinge stabilization mutation that specifically reacts with programmed cell death protein 1 (PD-1), also known as CD279. Sintilimab is shown to have a greater affinity for human PD-1 compared to both nivolumab and pembrolizumab. Sintilimab also shows cross-reactivity to PD-1 cynomolgus monkey species. PD-1 is an inhibitory receptor belonging to the CD28 family of the Ig superfamily, and it is transiently expressed on the cell surface of CD4 and CD8 T cells, B cells, as well as myeloid cells. PD-L1 is the main ligand of PD-1 and is expressed on the surface of tumor cells and antigen-presenting cells (APCs). PD-1 and PD-L1 interactions are central to immune homeostasis and prevention of autoimmunity. PD-1 binding to PD-L1 inhibits T cell activation, thereby diminishing T cell proliferation, cytokine production, and cytotoxic activity. This phenomenon is critical in the downregulation of the immune response during chronic inflammation and minimizing tissue damage. Cancers, such as squamous cell carcinoma, lung cancer, melanoma, ovarian cancer, renal cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma, often express PD-L1 in high abundance, wherein the cancer cells exploit the PD1/PD-L1 pathway to evade immune surveillance via immune evasion. In immune checkpoint inhibitor-responsive experimental models, the tumor growth can be transiently arrested in vivo through treatment with anti-PD-1 antibodies, e.g., sintilimab, which block the interactions between PD-1 and PD-L1 proteins.参数
| 同种型 | Human IgG4, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Human PD-1 |
| Reported Applications | in vivo functional assays in vitro functional assays Flow cytometry ELISA |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | <0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | >95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 提纯 | Protein A |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
推荐产品
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Recommended Isotype Control(s)
RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
