InVivoSIM anti-human VISTA (Onvatilimab Biosimilar)
货号 #SIM0057
克隆号:
Onvatilimab
反应种属:
Human
￥2,448 - ￥84,656
产品详情
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Onvatilimab, making it ideal for research use. Onvatilimab is a humanized IgG1 monoclonal antibody that reacts with V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA), also known as VSIR or B7-H5. VISTA is an immunoregulatory receptor that inhibits the T-cell response. VISTA is also involved in the stimulation of MMP14-mediated MMP2 activation. VISTA is suggested to promote embryonic stem cell differentiation through its inhibitory effects on BMP4 signaling. VISTA is a type I transmembrane glycoprotein from the Ig superfamily, and it is expressed in several myeloid cells, including CD11b monocytes, CD66b+ neutrophils, at low levels on CD4+ and CD8+ T-cells, and in a subset of NK cells. VISTA functions as a negative immune-checkpoint protein that suppresses T cell cytokine production and proliferation. VISTA is overexpressed by tumor-infiltrating lymphocytes, such as myeloid cells and regulatory T cells. In tumors, VISTA regulates T cell function, thus promoting antitumor effects. In preclinical mouse models, VISTA blockade with monoclonal antibodies decreases the MDSC infiltration in tumors with concomitant induction of immune effector infiltrates, thereby exerting positive effects on the survival of tumor-bearing mice.参数
| 同种型 | Human IgG1, κ |
|---|---|
| 推荐的同型对照 | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| 推荐稀释缓冲液 | InVivoPure pH 7.0 Dilution Buffer |
| 免疫原 | Human VISTA |
| Reported Applications | in vitro blocking of VISTA signaling in vivo blocking of VISTA signaling Functional assays Flow cytometry ELISA |
| 形式 | PBS, pH 7.0 Contains no stabilizers or preservatives |
| 内毒素 | <0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay |
| 聚合 | <5% Determined by SEC |
| 纯化 | >95% Determined by SDS-PAGE |
| 无菌性 | 0.2 μM filtration |
| 生产 | Purified from cell culture supernatant in an animal-free facility |
| 提纯 | Protein A |
| 分子量 | 150 kDa |
| 小鼠病原体试验 | Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Minute Virus: Negative Mouse Hepatitis Virus: Negative Reovirus Screen: Negative Lymphocytic Choriomeningitis virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Mouse Rotavirus: Negative Theiler's Murine Encephalomyelitis: Negative Ectromelia/Mousepox Virus: Negative Hantavirus: Negative Polyoma Virus: Negative Mouse Adenovirus: Negative Sendai Virus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Mouse Cytomegalovirus: Negative K Virus: Negative |
| 保存 | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
推荐产品
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Recommended Isotype Control(s)
RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
